Dormanomics Bulk RNA-seq Analysis

Transcriptome-wide gene expression profiling for dormant species.
Supporting differential expression, pathway enrichment, and isoform-level analysis across torpor-arousal cycles.

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Dormancy Focus: Time-series DEG analysis across torpor-arousal cycles, circadian rhythm disruption, cold-inducible RNA binding proteins, and seasonal metabolic switching.
1
Batch Correction & QC
ComBat-seq RIN > 7 Seasonal Factor
2
Time-series DEG Analysis
MASTS/Limma 5 Stages Trend Clustering
3
Rhythmicity & Pathway
Cosinor GSEA OXPHOS Score
4
Cross-tissue Integration
Circos Liver-Brain Axis Ketone Metabolism
Recommended Tools
ComplexHeatmap GSEA Plot Circos Boxplot Volcano Plot Venn Diagram